Agappe Dengue Combi device is an immunochromatographic assay kit for qualitative detection of NS1 Ag and differential detection of immunoglobulin G (lgG) and immunoglobulin M (lgM) against all types of dengue viruses using human serum, plasma or whole blood. In Dengue NS 1 Ag strip, dengue-specific antibodies complexed with gold conjugate are placed in the conjugate pad and anti-dengue NS1 antibodies are immobilized on the membrane. When dengue antigen-positive specimen is loaded into sample well, the antigen is captured by the immobilized antidengue NS1 antibodies. And then, the antigen is reacted with dengue-specific antibodies-gold complex to make visible band in the test line. The solution continues to migrate to encounter the control line antibody that binds a control conjugate, thereby producing a second red line. In Dengue IgG/IgM strip, antigen complexed with gold conjugate is placed in the conjugate pad and anti-human lgG and anti-human lgM are coated on the membrane. When dengue antibody-positive specimen is loaded into sample well, the antibodies are captured by the immobilized anti-human antibodies. And then, the antibodies are reacted with dengue-specific antigen-gold complex to make a visible band in the test line regions. The solution continues to migrate to encounter the control line antibody that binds a control conjugate, thereby producing a second red line. Agappe Dengue combi device can detect the antigen & antibodies against all serotypes of dengue virus.